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UniQ PINP for the USA

The UniQ Bone & Tissue Markers are unique serum tests for measuring collagen metabolism in bone and soft tissues.

The quantitative UniQ PINP RIA measures intact aminoterminal propeptide of type I procollagen liberated to blood stream during bone formation.

Generally

PINP - aminoterminal propeptide of type I procollagen - is a specific type I collagen metabolite which is liberated into the bloodstream during formation of new bone. Its concentration is directly related to the rate of bone turnover. In metabolic bone diseases such as osteoporosis, PINP is a sensitive indicator for assessing increased bone turnover in postmenopausal women. PINP is a useful parameter for monitoring the effectiveness of anti-resorptive and anabolic treatment and can be used as an aid in the management of postmenopausal osteoporosis.

UniQ PINP test provides you with

A specific and sensitive means of assessing the metabolism of bone.

PINP

  • Monitoring of the effect of osteoporosis treatment
  • Selecting the most effective treatment
  • Monitoring patient compliance
  • Changes in bone turnover seen in a couple of months.

Test results should never be used alone, without a complete clinical evaluation. Read more: UniQ PINP for the USA: Risks

Technical data

Products available
Use For in vitro diagnostic use
Method

Radioimmunoassay

Sample type Serum
Time to result Appr. 4 h
Reading of the result Gamma counter
Sensitivity

Appr. 2 µg/l

Specificity

2.7% cross-reactivity with small monomeric degradation products (col1), no cross-reactivity with PIIINP.

Storage 2 - 8 °C (36 - 46 °F)
Additionally needed

Gamma counter

Registered trademark UniQ is a registered trademark of Orion Diagnostica Oy.

About the tests

Collagen is the most common protein in the human body providing structural support to bone, skin, muscle and tendons. An indicator of type I collagen turnover, such as PINP, is useful for investigating skeletal remodelling under normal and abnormal conditions. 

PINP measurements provide a sensitive tool for assessing increased bone turnover in postmenopausal women and may be particularly useful for monitoring the effect of antiresorptive and anabolic therapy on bone metabolism.

More information

UniQ PINP kit is intended to be used as an aid in the management of postmenopausal osteoporosis. Short-term changes in PINP are associated with longer-term changes in bone mineral density in postmenopausal women. Following PINP concentrations during osteoporosis treatment provides a rapid assessment of a patient’s response to osteoporosis therapy.

Documents and materials

Marketing and sales materials

UniQ PINP RIA Data Sheet (GB)

UniQ PINP RIA Application Sheet Monitoring Osteoporosis

Instructions for use

(For informative use only. Kindly always refer to the latest package insert in the kit.)

UniQ PINP RIA IFU (GB), 67034

Risks and limitations of the procedure

Results of this assay should be used in conjunction with other pertinent clinical information for making diagnostic and therapeutic decisions. Therefore, the UniQ PINP assay is not recommended for use as a screening procedure to detect the presence of osteoporosis in the general population.

When evaluating subsequent samples, collect the samples at the same time of the day, as there is a diurnal variation of PINP with the values being higher at night.

Type I collagen is also synthesised by fibroblasts and therefore can be found in loose connective tissues together with other collagen types. However, since bone is the major collagenous organ and also metabolically active throughout life, the majority of the circulating PINP is of osteoblast origin.(1)

PINP is metabolised in the liver.(2) Severe liver disease may affect clearance from the circulation and give rise to elevated serum PINP levels.(3)

As with most assays utilising secondary antibodies, samples containing antibodies (such as heterophilic antibodies) that react with rabbit/goat IgG may cause erroneous results.

1. Risteli J and Risteli L. Products of bone collagen metabolism. In: Seibel MJ, Robins SP, Bilezikian JP(eds). Dynamics of bone and cartilage metabolism. Academic Press, San Diego, 1999. p 275-287.

2. Melkko J et al. Clearance of the aminoterminal propeptides of types I and III procollagen is a physiologicalfunction of the scavenger receptor in liver endothelial cells. J Exp Med 1994;79:405-412.

3 Naylor KE and Eastell R. Measurement of biochemical markers of bone formation. In: Seibel MJ, RobinsSP, Bilezikian JP (eds). Dynamics of bone and cartilage metabolism. Academic Press, San Diego, 1999. p401-410.

Frequently asked questions

Are there any substances in blood that can react on the UniQ PINP assay?
The only substances in blood that could be expected to react on the PINP assay are other peptide fragments. The cross-reactivities with the UniQ PINP assay have been tested and found to be non-significant. The PINP assay only detects the intact form of the propeptide, and does not cross-react with smaller degradation fragments such as Col-1.

What kind of test tubes should be used when running the UniQ PINP kit?
We recommend using polystyrene test tubes.

How is the collagen marker cleared from the circulation? Are there any conditions which can affect their clearance in the patient?
PINP is cleared from the circulation through a specialised part of the reticulo-endothelial system, the endothelial cells of the liver. These cells bind and internalise the propeptide by receptor-mediated endocytosis using so called scavenger receptors. Severe liver disease can affect the clearance of PINP. As renal insufficiency does not affect the concentration of the propeptide, it is very useful for monitoring changes in collagen metabolism in patients with renal failure.

What is the best way for result processing with the UniQ PINP assay?
The best way to process the results is to use spline function curve fitting with linear y-axis and logarithmic x-axis.

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