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Uricult tests

Uricult culture tests are intended for diagnosing urinary tract infections (UTI) by demonstration and presumptive detection of microbes (total bacterial count, gram-negative bacteria, enterococci, E. coli) in urine samples. The test can be performed at the point of care, or the slides can be used as convenient transport media for cultured samples.

Generally

Uricult tests are available in three different, easy-to-use versions.

The Uricult dipslide is based on two agar media. The CLED agar is intended for determining the total bacterial count in urine samples and the selective MacConkey agar supports the growth of gram-negative bacteria.

The Uricult Plus dipslide is based on three agar media. In addition to the CLED and MacConkey agar media, Uricult Plus has a selective Enterococcus medium which is intended specifically for the detection of enterococci.

The Uricult Trio dipslide is based on three agar media. In addition to the CLED and MacConkey agar media, Uricult Trio has a selective E.coli medium which is intended specifically for the detection of gram-negative, β-glucuronidase-producing organisms, of which Escherichia coli is the most common in urinary tract infections.

Uricult tests provide you with

  • On-site tests eliminating the need for transporting urine samples
  • The result gives an exact picture of the clinical state at the time of sampling
  • Easy-to-perform: Dip - Incubate - Interpret
  • Durability, as the Uricult slides can be stored at room temperature for months

Test results should never be used alone, without a complete clinical evaluation.

Uricult is not registered in the USA.

Technical data

Products available
Use For in vitro diagnostic use
Method

Culture

Sample type Urine
Time to result 16 - 24 hours
Reading of the result Visual
Storage 7 - 25 °C
Additionally needed

Incubator capable of +36 ± 2 °C

Registration Not registered in the USA
Note

Store in an area protected from air and temperature fluctuations. Do not allow to freeze.

Registered trademark Uricult is a registered trademark of Orion Diagnostica Oy.

About the tests

Urinary tract infection (UTI) is one of the most common infections caused by bacteria. It is important to verify and treat UTI adequately since untreated UTI may cause kidney damage. Bacterial culture of an appropriately collected and handled urine sample can provide an accurate diagnosis. However, transportation of urine samples from the point of care site to a laboratory may lead to incorrect results as bacteria may multiply if the samples are not transported correctly.

The Uricult tests are dipslides for culturing urine samples. The slides are covered on both sides with agar media, two or three media depending on the test. The slide can be dipped into urine, or urine can be poured onto the slide. Thereafter, the inoculated slide is inserted back into the tube, making it ready for incubation or for transportation. After incubation, the presence of bacteria is evidenced by colonies growing on the agar surface. Because each colony is the result of multiplication of a single bacterial cell, the number of colonies indicates the number of colony forming units (CFU/ml) in the urine sample.

The total colony count on all Uricult tests is determined from the originally green CLED agar by matching the colony density with the model chart. The bacteria may be classified as lactose positive or negative based on their appearance on CLED agar. The number of gram-negative bacteria can be determined for all Uricult tests from the originally brownish–red, selective MacConkey agar.

In the Uricult Plus assay, Enterococci and some group B streptococci produce colonies in various shades of red onto the third, colourless, selective enterococcus agar. This special agar is a chromogenic agar which makes the interpretation of results even easier.

In the Uricult Trio assay, β-glucuronidase-producing organisms form colonies in various shades of brown or grey onto the third, colourless, selective E.coli agar. This special agar is a chromogenic agar which makes the interpretation of results even easier. Up to 4 out of 5 UTI in primary care patients are caused by E.coli.

Documents and materials

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