Urinary tract infection (UTI) is one of the most common infections caused by bacteria. It is important to verify and treat UTI adequately since untreated UTI may cause kidney damage. Bacterial culture of an appropriately collected and handled urine sample can provide an accurate diagnosis. However, transportation of urine samples from the point of care site to a laboratory may lead to incorrect results as bacteria may multiply if the samples are not transported correctly.
The Uricult tests are dipslides for culturing urine samples. The slides are covered on both sides with agar media, two or three media depending on the test. The slide can be dipped into urine, or urine can be poured onto the slide. Thereafter, the inoculated slide is inserted back into the tube, making it ready for incubation or for transportation. After incubation, the presence of bacteria is evidenced by colonies growing on the agar surface. Because each colony is the result of multiplication of a single bacterial cell, the number of colonies indicates the number of colony forming units (CFU/ml) in the urine sample.
The total colony count on all Uricult tests is determined from the originally green CLED agar by matching the colony density with the model chart. The bacteria may be classified as lactose positive or negative based on their appearance on CLED agar. The number of gram-negative bacteria can be determined for all Uricult tests from the originally brownish–red, selective MacConkey agar.
In the Uricult Plus assay, Enterococci and some group B streptococci produce colonies in various shades of red onto the third, colourless, selective enterococcus agar. This special agar is a chromogenic agar which makes the interpretation of results even easier.
In the Uricult Trio assay, β-glucuronidase-producing organisms form colonies in various shades of brown or grey onto the third, colourless, selective E.coli agar. This special agar is a chromogenic agar which makes the interpretation of results even easier. Up to 4 out of 5 UTI in primary care patients are caused by E.coli.