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SIBA® article on Influenza A and B published in Applied Microbiology and Biotechnology

New scientific article on SIBA technology is now available online. The name of the article is Reverse transcription strand invasion based amplification (RT-SIBA): a method for rapid detection of influenza A and B.

The article, published online in Applied Microbiology and Biotechnology (publisher: Springer) on the 11th of April 2016, describes the development of a variant of the SIBA method, namely, reverse transcription SIBA (RT-SIBA), for the rapid detection of viral RNA targets. The RT-SIBA method includes a reverse transcriptase enzyme that allows one-step reverse transcription of RNA to complementary DNA (cDNA) and simultaneous amplification and detection of the cDNA by SIBA under isothermal reaction conditions. The RT-SIBA method was found to be more sensitive than PCR for the detection of influenza A and B and could detect 100 copies of influenza RNA within 15 min. The development of RT-SIBA will enable rapid and accurate diagnosis of viral RNA targets within point-of-care or central laboratory settings.

Link to the article:

Orion GenRead® is based on SIBA technology. Read more about Orion GenRead