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New peer reviewed publication: RT-SIBA assay for detection of respiratory syncytial virus
Respiratory syncytial virus (RSV) is one of the most common causes of respiratory tract infections among young children and elderly. The article describes a rapid reverse transcription strand invasion based amplification (RT-SIBA) assay for detection of RSV from nasopharyngeal (NP) swabs. The analytical sensitivity of the RT-SIBA assay was comparable to a RT-PCR assay. As few as 10 copies of RSV RNA were detected with RT-SIBA within 20 minutes while RT-PCR took approximately 2 hours.
Clinical samples analysed during the study were rapidly processed without RNA extraction steps. The sample preparation method used in this study takes only about 5 minutes while traditional RNA extraction can take up to 45 minutes. The RT-SIBA results for the RSV positive clinical samples were available in 15 to 44 minutes while the detection time for RT-PCR can be up to 2 hours. This crude sample preparation method combined with the rapid RT-SIBA assay could shorten the time to detect RSV from NP swab specimens when compared to traditional RT-PCR methods. RT-SIBA displayed a detection time as rapid as that of rapid antigen detection tests (RADTs) and a high analytical sensitivity similar to that of RT-PCR.
The article Rapid and sensitive real-time assay for the detection of respiratory syncytial virus using RT-SIBA® was published in BMC Infectious diseases on 10th of February 2017.
Eboigbodin et al. BMC Infectious Diseases (2017) 17:134 Doi: 10.1186/s12879-017-2227-x
Link to the article: https://bmcinfectdis.biomedcentral.com/articles/10.1186/s12879-017-2227-x