The upper part of the Uricult Vet culture medium has shrunk by approximately 1.5 mm. Is it still usable?
You can still use the slides if the medium has not shrunk more than that.
I received Uricult Vet vials where one medium is separated from the dipslide. Why does the medium separate?
The agars may become loose if the boxes are not handled with care and if the boxes have been dropped on the floor. Freezing may also cause agars to fall off. If the agar has been frozen, it will typically have an "orange-like" surface. Although freezing is more likely to happen during transportation in winter, it may also occur during summer. Storage too close to the cooling system or in a too cold refrigerator may freeze the agar.
When opening the Uricult Vet kit I saw that the media had dried out with cracks on the surface and some were contaminated. Why does this occur?
The description above (a dry and cracked surface) strongly suggests that the slides have become frozen at some point, either during transportation or storage. Noticing contaminated slides in the same box may also be a sign of freezing as the frozen surface becomes dull and may look like it has been heavily colonised. If you notice separate colonies, it is probably due to contamination. Contamination cannot be completely avoided because of our aseptic but not sterile manufacturing process. To avoid any contaminated slides being packed into kits, we do a visual inspection before packaging. In case of contamination, we always inspect our retain stock to exclude this possibility.
I received a box of Uricult Vet today and all the bottles have pink liquid inside and a lot of small liquid bubbles on the wall of the bottle. Is that normal?
Uricult Vet contains water in the agar. If there is a temperature fluctuation or draught, it may result in water evaporation. This can be seen as liquid in the tube and also as small drops on the tube walls. The colour of the liquid is a result of the colour of the agar. It is a normal phenomenon and should not affect the use of Uricult Vet as long as it has not resulted in drying of the agar.
Uricult Vet has two media on it, which are these and why are they used?
Uricult Vet has CLED medium on one side and MacConkey medium on the other side. All bacteria should be able to grow on the green CLED medium which is intended for estimating the total bacterial count. Lactose fermenting bacteria will grow in yellow colonies, and they will also change the colour of the medium from green to yellow. Non-lactose fermenting bacteria grow on CLED medium as colourless colonies. Gram-negative bacteria mainly grow on the brownish MacConkey medium, although some gram-positive bacteria may also be able to grow there. On this medium, lactose fermenting bacteria grow in red colonies, whereas non-lactose fermenting bacteria grow as colourless colonies.
Can Uricult Vet be stored at 15 - 25 °C or at 2 - 8 °C? What are the deviation limits? How long can it be kept outside that range? Can it be frozen?
Uricult Vet is best preserved if it is stored at 7 - 25 °C. The products must not be frozen. Extra care should be taken to avoid draughts and temperature fluctuations, which may cause the agar to dry, thereby shortening the shelf-life.