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SIBA® (Strand Invasion Based Amplification) is Orion Diagnostica’s proprietary isothermal nucleic acid amplification technology. The technology is based on amplification of nucleic acids at a constant temperature of 40 degrees Celsius.

Please click the area below to see how the SIBA technology works:



Documents and materials

SIBA licensing and partnering opportunities (EN)

Scientific articles

Hoser MJ, Mansukoski HK, Morrical SW, Eboigbodin KE (2014) Strand Invasion Based Amplification (SIBA®): A Novel Isothermal DNA Amplification Technology Demonstrating High Specificity and Sensitivity for a Single Molecule of Target Analyte. PLoS ONE 9(11): e112656. doi:10.1371/journal.pone.0112656A 


Eboigbodin KE & Hoser MJ (2016). Multiplex Strand Invasion Based Amplification (mSIBA) assay for detection of Chlamydia trachomatis and Neisseria gonorrhoeae. Scientific Reports, 6, 20487. Doi: 10.1038/srep20487 


Eboigbodin K, Filén S, Ojalehto T, Brummer M, Elf S, Pousi K & Hoser M. Reverse transcription strand invasion based amplification (RT-SIBA): a method for rapid detection of influenza A and B Applied Microbiology and Biotechnology, 2016, April 11. Doi: 10.1007/s00253-016-7491-y.


Eboigbodin KE, Brummer M, Ojalehto T & Hoser M (2016). Rapid molecular diagnostic test for Zika virus with low demands on sample preparation and instrumentation. Diagnostic microbiology and infectious disease, 86, 369-371. https://doi.org/10.1016/j.diagmicrobio.2016.08.027


Eboigbodin KE, Moilanen K, Elf S & Hoser M (2017). Rapid and sensitive real-time assay for the detection of respiratory syncytial virus using RT-SIBA®. BMC Infectious Diseases, 17, 134. Doi: 10.1186/s12879-017-2227-x.



SIBA Poster ESCV 2017 (EN)

SIBA Poster MDE 2016 (EN)

SIBA Poster EMMD 2015 (EN)

SIBA Poster RME 2015 (EN)


Orion GenRead is based on the SIBA technology. Read more about Orion GenRead.